Cannabinoid Profiling by High Pressure Liquid Chromatography (HPLC)

This analysis includes: delta-8 THC, delta-9 THC, THCA, THCV, CBD, CBDA, CBDV, CBN, CBG, CBGA

Cannabinoid profiling (potency) testing via liquid chromatography is required for all products sold in California and includes moisture analysis. Many of our clients use HPLC testing to accurately dose their infused products, gauge the completeness of their decarboxylation process. This method uses solvents rather than heat during the analysis, allowing for detection of non-psychoactive, acidic Cannabinoids like THCA, CBDA, and CBGA, which form THC and CBD when heated.

 

 

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Microbiological Analysis via FDA BAM & Mycotoxin Analysis via LC-MS/MS

Is my product clean? How many bacteria or fungi are present in my sample? Are there dangerous pathogens in my sample?

CW Analytical has the experience and capability to screen for all microbiological impurities required by the State of California including: Shiga-toxin producing E.coli, Salmonella sp., and Aspergillus flavus, niger, fumigatus, and terreus. We also have the ability to test for aerobic bacteria, yeast, mold, and other contaminants in your medical Cannabis product.

Mycotoxin testing is required by the state of California for all Cannabis and Cannabis products cultivated or manufactured in 2019. Samples are tested for aflatoxin B1, B2, G1, and G2, and ochratoxin A via liquid chromatography–tandem mass spectrometry. These mycotoxins are secondary metabolites produced by microfungi and some mold, and can cause serious disease when consumed.

Chemical Residue (Pesticide) Testing by LC-MS/MS & GC-MS/MS

Are there residues of pesticides or fungicides in my product?

The State of California requires all Cannabis products sold to be screened for residual pesticides. Many pesticides can be harmful for human consumption, and some pesticides commonly found in Cannabis are only approved for ornamental plants.

CW can currently quantify the concentration of 66 different pesticides including all compounds on Category 1 & 2 Lists required for CA state compliance. Acceptable limits vary depending on compound and sample type (see BCC section 5719).

Terpene Analysis by GC-FID

What compounds are contributing to the smell or flavor profile of my sample? What are the concentrations of terpenes in my sample?

Terpenes are a specific class of organic chemical compound found in Cannabis that contribute strongly to the smell and flavor of the flower, but may also have additional medical benefits.

CW tests and quantifies 21 different terpenes, including Myrcene, Limonene, Caryophyllene, and many others.

A comprehensive list can be found here.

Residual Solvent Testing via GC-FID

Is my sample free of residual solvents leftover from extraction? (e.g. Butane, Propane, Alcohol)

Many Cannabis concentrate producers utilize a variety of solvents to manufacture high-quality, potent, waxes and oils. While many of these commonly used solvents (ethanol, isopropyl, butane, propane) carry G.R.A.S status (generally regarded as safe) it's important to understand the constituents of any product patients are consuming.

CW can currently quantify the concentration of 20 different solvents including all compounds on Category 1 & 2 Lists required for CA state compliance. Acceptable limits vary depending on compound and sample type (BCC section 5718).

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Moisture Content & Water Activity

Is there too much moisture in my product? Is my product susceptible to bacteria, yeast, and fungal growth?

Water activity is defined as the quantity of water in a product that is available and therefore capable of supporting bacteria, yeasts, and fungi. Water activity should not be confused with the water content - the “chemically bound” water - of a product. Water activity influences a product’s microbiological and chemical stability. 

Water activity testing is required by the state of California for Cannabis and solid edible Cannabis products cultivated or manufactured in 2019 (BCC section 5717).

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Heavy Metal Analysis by ICP-MS

Does my product contain cadmium, lead, arsenic, or mercury?

Samples are tested via Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) for cadmium, lead, arsenic, and mercury, which can be toxic when consumed. These heavy metals can potentially be present in the Cannabis plant from soil uptake, fertilizer, water, hydroponic media, or environmental pollution.

Heavy metal testing is required by the state of California for all Cannabis and Cannabis products cultivated or manufactured in 2019 (BCC section 5723).

Consulting

If you have further questions or needs beyond our suite of tests, we're glad to help!

Sometimes the needs of our clients go beyond what our typical tests can provide, and that's perfectly fine. CW Scientists have decades of experience in the food sciences, microbiology, chemistry, and Cannabis, and can help you refine and optimize your product.  If we have the capability to help, we will! Contact CW Analytical to inquire about consultations and our hourly rate.